SOLIScript® 1-step Probe Kit (08-57-00250)
Price: Contact
Brand: Solis BioDyne / Origin: Estonia
Storage: -20 °C
Shipping: Stable up to 1 month at room temperature
| Catalogue number | Size |
08-57-0000S |
50 rxn | 50 x 20 μl rxn free sample |
08-57-00250 |
250 rxn | 250 x 20 μl rxn |
Simple and highly specific cDNA synthesis and qPCR in a single tube
Description
SOLIScript® 1-step Probe Kit is optimized for probe based one-step RT real-time quantitative PCR assays.
It contains all the components necessary (except template, primers, and probes) to perform cDNA synthesis and probe based qPCR in a single tube
Reagents
5x One-step Multiplex Probe Mix (ROX) with HOT FIREPol® DNA polymerase, reaction buffer, dNTPs including dUTP), 15 mM MgCl2 (1x qPCR solution – 3 mM MgCl2), internal reference based on ROX dye
40x One-step SOLIScript® Mix with SOLIScript® Reverse Transcriptase and RiboGrip™ RNase Inhibitor
Water, nuclease free
Properties
Sample type: RNA
Product format: Reagent kit
No of tubes: three tubes
Detection type: Probe-based
Reference dye: reference dye based on ROX
Compatible real-time instruments: The Kit is compatible with most qPCR cyclers that do or do not require an internal reference dye (e.g. ROX) for normalization of fluorescent signal.
Applications
- Detection and quantification of RNA targets with qPCR
- DNA/LNA hydrolysis probe based assay
- Suitable for singleplex or duplex reactions
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