SolisFAST® Probe qPCR Mix with UNG (28-22-00001)

Fast, highly sensitive and reproducible probe-based qPCR solution

Description

SolisFAST^® Probe qPCR Mix is a 5x-concentrated, ready-to-use solution for fast, highly sensitive and reproducible probe-based qPCR assays using dual-labeled hydrolysis probes (e.g. TaqMan^® probes), and is suitable for detection and quantitation of up to five targets simultaneously.

Combining novel in silico-designed SolisFAST^® DNA Polymerase with fast hot-start and optimized buffer, this qPCR Mix offers accurate target detection and quantification.

For extra convenience the mix includes dUTP and UNG enzyme that enable UNG treatment to prevent carryover comtamination.

This master mix is developed for TaqMan^® probes but is also suitable for other hydrolysis probe types. 

Mix Components:

SolisFAST^® DNA Polymerase: In silico- designed analogue of Taq DNA polymerase with enhanced stability at room temperature, fast hot-start and faster extension rates compared to the wild-type Taq DNA polymerase.

qPCR buffer: Includes 16.5 mM MgCl₂ (1x PCR solution 3.3 mM MgCl₂), dNTPs (dATP, dCTP, dGTP, dUTP), UNG, additives that maximize efficiency of PCR 

Properties

Concentration: 5x

Hot-start: yes, 3 minutes initial incubation at 95°C

Cycling speed: fast or ultra-fast

Detection type: Probe-based

Reference dye: none

Multiplex: up to 5 plexes simultaneously

Compatible real-time instruments: The mix is compatible with qPCR cyclers that do not require an internal reference dye (e.g. ROX) for normalization of fluorescent signal.

Applications

• DNA/LNA hydrolysis probe based assays
• Detection and quantification of DNA and cDNA targets
• Profiling gene expression
• Microbial detection
• Viral load determination

ABT Equipment Co., Ltd is proud to be the official distributor of Meridian Bioscience – Bioline. If you have any questions or need further assistance, please contact: