Introduction
Early Mortality Syndrome (EMS), also known as Acute Hepatopancreatic Necrosis Syndrome (AHPNS), is a disease that seriously affects the shrimp farming industry in Vietnam. The disease was first reported in China in 2009 and is thought to be caused by Vibrio parahaemolyticus that resides in the digestive tract of shrimp by a rather complex mechanism. Manifestations in the early stages are often not obvious: slow-growing, lethargy, decreased appetite, spinning on the water surface. Shrimp die very quickly, only about 2-3 days after detecting the disease. Therefore, it is extremely necessary to detect and prevent the spread of diseases, in order to minimize the damage.
” TOPSPEC ® AHPND/EMS REALTIME PCR KIT (SQS-152) is a PCR-based test kit that offers fast and accurate detection of EMS agent in shrimp. The average time for EMS agent detection is 2 hours. The kit applies next-generation Hot Start Taq Polymerase technology and an optimized buffer system to improve performance, sensitivity and reaction time. In addition, during extraction or mixing, exogenous controls can be added to avoid false negatives and manipulation errors.”
Advantages of the kit
- Simple process
- PCR optimal time: 2 hours
- Various types of input samples
- High sensitivity and specificity
- Provide additional master mix for control test (75 tests)
- Compatible with most real-time PCR machines on the market: Rotor Gene Q (Qiagen), 7500, 7500 Fast (Thermo Fisher), AriaMX, Mx 3005p, Bioer, Dlab, etc.
Specifications
Target | AHPND/EMS |
Sample | DNA after extraction from shrimp samples |
Volume | 5µL |
Channel | FAM: AHPND/EMS
HEX: internal control |
Technology | TaqMan probe |
PCR time | 2 hours |
Specificity | Kit only detects AHPND/EMS |
Components | HPND/EMS qPCR mix, Negative control, Positive control, Internal control (IC), Tube PCR. |
Storage | 12 months, temperature -20oC |
Manual | User Manual |
Above is detailed information about TopSPEC ® AHPND/EMS REALTIME PCR KIT (SQS-152.1). In addition, we also have other detection kits such as AHPND, EMS, DIV1, etc.
See more products: >>> AQUATIC DISEASES DETECTION KIT<<<
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