Introduction
Muscle necrosis in shrimp is an infectious disease caused by the infectious myonecrosis virus – IMNV. The first case was reported in northeastern Brazil in 2002, then spread to Asia. The virus usually parasitizes skeletal muscle, connective tissue, blood cells, and lymphatic organs. The disease usually appears on white leg shrimp with white opaque spots in the abdominal segments. Infected shrimp might have milky or orange abdominal muscles, and sometimes a simultaneous molt may appear. The mortality rate is 40-70%, however, in many cases, it can be as high as 100%. The disease has no specific treatment, so early prevention and detection play an important role in minimizing loss for shrimp farmers.
” TopSPEC® IMNV RT-qPCR KIT (SQS-242) is a test kit based on Real-time PCR method that allows fast and accurate detection of IMNV agent in shrimp. The kit has the advantages of specificity, high sensitivity, endogenous control help ensure the extraction process or master mix. “
Advantages of the kit
- Simple process
- PCR optimal time: 2 hours
- Various types of input samples
- High sensitivity and specificity
- Provide additional master mix for control test (75 tests)
- Compatible with most real-time PCR machines on the market: Rotor Gene Q (Qiagen), 7500, 7500 Fast (Thermo Fisher), AriaMX, Mx 3005p, Bioer, Dlab, etc.
Specifications
Target | Infectious Myonecrosis Virus (IMNV) |
Sample | RNA after extraction from shrimp samples |
Volume | 5µL |
Channel | FAM: IMNV
HEX: internal control |
Technology | TaqMan probe; One-step RT-qPCR |
PCR time | 2 hours |
Specificity | Kit only detects IMNV |
Components | IMNV qPCR mix, Negative control, Positive control, Internal control (IC), Tube PCR. |
Storage | 12 months, temperature -20oC |
Manual | User Manual |
Above is detailed information about TopSPEC® IMNV RT-qPCR KIT (SQS-242). In addition, we also have other detection kits such as AHPND, EMS, DIV1, etc.
See more products: >>> AQUATIC DISEASES DETECTION KIT<<<
ABT Equipment Co., Ltd. is always confident and proud of our staff with years of experience in molecular biology, especially test kits related to viral and bacterial diseases in humans. If you have any questions or need help, please contact: