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    Specializing in scientific and technical equipment – tools – laboratory chemicals

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Trang chủ / Products / REALTIME PCR KIT / HUMAN DISEASE DETECTION KIT
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New product
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News
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  • Solution for diagnosis of hepatitis B (Hepatitis virus – HBV) in healthcare Chức năng bình luận bị tắt ở Solution for diagnosis of hepatitis B (Hepatitis virus – HBV) in healthcare
  • What you need to know about HPV (Human Papilloma Virus) Chức năng bình luận bị tắt ở What you need to know about HPV (Human Papilloma Virus)
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  • Taking next steps in Malaria control and prevention Chức năng bình luận bị tắt ở Taking next steps in Malaria control and prevention
Kit Xet Nghiem Hbv 1 300x300

TopSENSI® HBV qPCR KIT (RUO) (SQH-101)

Price: Contact

  • Accurate quantification of HBV virus concentration: using 5 standard points in combination with negative control, positive control, internal control system to detect abnormal results in a timely manner
  • High specificity: detect all HBV genotypes at the 37S protein gene.
  • High sensitivity: up to 25 IU/mL.
  • Code: QH01.HBV01A (50 test)
  • Detail
Introduction
Hepatis B Virus is a virus of the family Hepadnaviridae with a double-stranded DNA structure. It is estimated that 350 million people are chronically infected with HBV each year. Patients with chronic hepatitis can progress into cirrhosis and liver cancer. Therefore, accurate detection and quantification of HBV in the blood will make treatment for the patients easier.

” TopSENSI® HBV qPCR KIT (RUO) (SQH-101) is a test kit based on real-time PCR method that allows accurate detection and quantification of HBV concentration in human serum/plasma samples. The standard curve is built based on 5 points that give the most accurate quantitative results. The kit has the advantages of specificity, high sensitivity, endogenous control helps ensure the extraction process or master mix. ”


Advantages of the kit

  • Simple process
  • PCR optimal time:1.5 hours
  • High sensitivity and specificity (Ability to detect 250 IU/mL)
  • Supplied with a complete set of consumables
  • Provide additional master mix for control test (75 tests)
  • Compatible with most real-time PCR machines on the market: Rotor Gene Q (Qiagen), 7500, 7500 Fast (Thermo Fisher), AriaMX, Mx 3005p, Bioer, Dlab, etc.

Specifications

Target Hepatitis B Virus (HBV)
Sample DNA after extraction from initial serum and plasma samples
Volume 10µL DNA
Channel FAM for HBV and HEX for internal control (IC)
Sensitivity 250 IU/ ml
Specificity 100%
Calibration curve (E1: 10¹) (E2: 10²) (E3: 10³) (E4: 10⁴)(E5: 10⁵)
Technology TaqMan probe
PCR time 1.5 hours 
Components HBV qPCR mix, HBV standard (E1,E2,E3,E4,E5), Negative control, Internal control (IC), Tube PCR.
Storage 12 months, temperature -20oC
Manual >>>User Manual<<<

Quality Control

Figure 1: Evaluation of the HBV kit sensitivity, standard sample with 10^9 IU/mL concentration on Lightcycler 96 at a standard laboratory.
Figure 2: Evaluation of the repeatability of the HBV kit from 1 sample, 10 consecutive dilutions, each time was repeated 3 times on the LightCycler 96 at a standard laboratory.
Figure 3: Evaluation of quality between ABT kit (E1-E4) and R supplier (e1-e4), results were conducted on LightCycler 96 at a standard laboratory.
Figure 4: Result from the actual sample of patient A, HBV concentration 4.6×10^5 IU/mL, and calibration curve (E1-E5) on LineGene 9600 Plus.

Above is detailed information about TopSENSI® HBV qPCR KIT (RUO). In addition, we also have other detection kits such as HCV, HPV, MTB, NG-CHT, etc.

See more products: >>> HUMAN DISEASES DETECTION KIT<<<

ABT Equipment Co., Ltd. is always confident and proud of our staff with years of experience in molecular biology, especially test kits related to viral and bacterial diseases in humans. If you have any questions or need help, please contact:

Hotline: 0935 069 459
Email: support@abt-vn.com

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Contact information

ABT EQUIPMENT CO., LTD

  • Headquarter: No.275, Chien Thang Street, Van Quan Ward, Ha Dong District, Hanoi City, Vietnam
  • Representative office: A10-TT16, Van Quan – Yen Phuc New Urban Area, Van Quan Ward, Ha Dong District, Hanoi City, Vietnam
  • Laos: Nongbone Street, Xaysettha Ward, Vientiane, Laos
  • Phone number: (024) 7300 3386
  • Hotline: (+84) 935 069 459
  • Support: (+84) 327637845
  • Email: info@abt-vn.com
    Business code: 0107595864
    Register on October 13, 2016
    Issuing agency: Department of Planning and Investment of Hanoi
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  • Home
  • About Us
    • Enterprise Structure
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  • Service
    • Consulting Lab Setup – Standard Lab
  • User manual
  • Recruit
  • Blogs
  • Contact
  • Product Category
    • EXTRACTION KIT
      • SILICA COLUMN EXTRACTION KIT
      • CRUDE EXTRACTION KIT
      • MAGNETIC BEADS EXTRACTION KIT
    • REALTIME PCR KIT
      • HUMAN DISEASE DETECTION KIT
      • AQUATIC DISEASES DETECTION KIT
      • ANIMAL DISEASE DETECTION KIT
      • FOODBORNE DISEASE DETECTION KIT
      • GMO KIT
    • PCR KIT
      • AQUATIC DISEASE DETECTION KIT
    • LABORATORY CHEMICAL
      • MICROBIOLOGICAL ASSAY CHEMICALS
      • MOLECULAR BIOLOGY CHEMICALS
      • OTHER LABORATORY CHEMICALS
    • LABORATORY EQUIPMENT
      • LABORATORY INSTRUMENTS
        • MAGNETIC STIRRER
        • LABORATORY SHAKER
        • LABORATORY CENTRIFUGE
        • DRY BATH
      • OTHER EQUIPMENT
      • MOLECULAR BIOLOGY EQUIPMENT
        • AUTOMATIC GEL IMAGING SYSTEM
        • ELECTROPHORESIS EQUIPMENT
        • PCR MACHINE
        • SPECTROPHOTOMETER
        • MÁY REALTIME PCR
        • MÁY TÁCH CHIẾT TỰ ĐỘNG
    • COMSUMABLE SUPPLIES
      • OTHER INSTRUMENTS
      • FILTER TIP
      • PCR TUBE
      • REAL-TIME PCR TUBE
      • CENTRIFUGE TUBE
    • GENE SEQUENCING
    • PRIMER SYNTHETIC

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