TopSENSI® HCV qPCR Kit (RUO) (SQH-102)
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- Accurate quantification of HCV virus concentration: using 5 standard points in combination with negative control, positive control, internal control system to detect abnormal results in a timely manner
- High specificity: detects the HCV genotype at HCV73 5′ UTR.
- High sensitivity: up to 250 IU/mL.
- Code: QH02.HCV01A (50 test)
Introduction
Hepatis C Virus is a silent killer, with a much higher rate of complications (like cirrhosis and liver cancer) than hepatitis B virus. HCV has a single-stranded RNA structure. It is estimated that each year about 3% of the population gets hepatitis and about 170 million people are infected with the hepatitis C virus. Currently, HCV has no specific treatment, so early detection and accurate quantification will help to better the treatment for the patients.
TopSENSI® HCV qPCR Kit (RUO) (SQH-102) is a test kit based on real-time PCR method that allows accurate detection and quantification of HCV concentration in human serum/plasma samples. The standard curve is built based on 5 points that give the most accurate quantitative results. The kit has the advantages of specificity, high sensitivity, endogenous control helps ensure the extraction process or master mix.
Advantages of the kit
- Simple process
- PCR optimal time:1.5 hours
- RNA agent, available in both one-step and two-step real-time PCR (refer to TopSENSI cDNA synthesis Kit).
- High sensitivity and specificity (Ability to detect 250 IU/mL)
- Supplied with a complete set of consumables
- Provide additional master mix for control test (75 tests)
- Compatible with most real-time PCR machines on the market: Rotor Gene Q (Qiagen), 7500, 7500 Fast (Thermo Fisher), AriaMX, Mx 3005p, Bioer, Dlab, etc.
Specifications
| Target | Hepatitis C Virus (HCV) |
| Sample | DNA after extraction from initial serum and plasma samples |
| Volume | 10µL DNA |
| Channel | FAM for HCV and HEX for internal control (IC) |
| Sensitivity | 250 IU/ ml |
| Specificity | 100% |
| Calibration curve | (E1: 10¹) (E2: 10²) (E3: 10³) (E4: 10⁴)(E5: 10⁵) |
| Technology | TaqMan probe |
| PCR time | 1.5 hours |
| Components | HCV qPCR mix, HCV standard (E1,E2,E3,E4,E5), Negative control, Internal control (IC), Tube PCR. |
| Storage | 12 months, temperature -20oC |
| Manual | >>>User Manual<<< |
Quality control
Above is detailed information about TopSENSI® HCV qPCR KIT (RUO) (SQH-102). In addition, we also have other detection kits such as HCV, HPV, MTB, NG-CHT, etc.
See more products: >>> HUMAN DISEASES DETECTION KIT<<<
ABT Equipment Co., Ltd. is always confident and proud of our staff with years of experience in molecular biology, especially test kits related to viral and bacterial diseases in humans. If you have any questions or need help, please contact:
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