Introduction
Human papillomavirus (HPV) is the causative agent of genital problems in humans and can lead to cervical cancer in women. High-risk HPV infection is the main cause of cervical cancer. It is estimated that Human papillomavirus affects up to 500,000 women and kills more than 270,000 each year. The disease usually progresses very slowly until it shows symptoms, which often lead to serious complications for women’s health, so early detection and screening for this virus is extremely necessary.
” TopCARE ® HPV qPCR Kit is a test kit based on the real-time PCR method that allows fast and accurate detection of HPV in human smears/biopsy samples. The kit has the advantages of specificity, high sensitivity, endogenous control helps ensure the extraction process or master mix. ”
Components
Components | Storage |
HPV qPCR Mix | -20oC |
Positive control | -20oC |
Negative control | Room temperature |
PCR Tube | Room temperature |
Feature
- Target: Human papillomavirus
- Input volume: 200 µL
- Starting materials: human smears/biopsy samples
- Detection threshold: 10 copies/reaction.
- Fluorescent Technology: TaqMan Probe.
- Channel: FAM – HPV agent; HEX – internal control.
- Manual extraction: TopPURE Genome DNA Extraction Kit.
- Automatic Extraction: TopPURE Maga Genomic DNA/RNA Extraction Kit.
- Work-flow: 2 hours 15 minutes.
- Storage: 12 months at -20oC.
- Residual chemicals volume is up to 75 tests/box.