INTRODUCTION
Classical Swine Fever is caused by the Classical Swine Fever Virus (CSFV) and is one of the most infectious and damaging diseases. The disease shows some first manifestations after 3-15 days, commonly causing fever, skin lesions, and convulsions. Infected piglets will die after 15 days. Mortality is high when infected with highly virulent viruses. Therefore, early screening and diagnosis measures should be prioritized.
TopSPEC® CSFV RT-qPCR Kit is designed to detect the presence of CSFV on various samples (tissues, organs, organs, whole blood, serum, and environmental surface samples) by Real-time PCR. The average time for the complete process of detection is 2 hours. The kit is a combination of many modern technologies in molecular biology to significantly improve the specificity and sensitivity of the reaction compared to other products on the market today. In addition, during extraction or mixing, exogenous controls can be added to limit false negatives and manipulation errors.
Advantage
- Easy to use
- PCR time optimized for 2 hours
- Various types of samples
- High sensitivity and specificity
- Provide additional mastermix for control (75 tests)
- Compatible with most Realtime PCR machines on the market: Rotor Gene Q (Qiagen), 7500, 7500 Fast (Thermo Fisher), AriaMX, Mx 3005p, Bioer, Dlab, etc.
Specification
Target | Classical Swine Fever Virus (CSFV) |
Sample | RNA after extraction from serum, oral fluid, lung tissue, lymph nodes or environmental samples of infected pigs |
Volume | 5µL |
Channel | FAM: for CSFV
HEX: for internal control |
Technology | TaqMan probe |
PCR time | 2 hours |
Specificity | only detect CSFV |
Components | CSFV rRT-PCR mix, Negative Control, Positive Control, Internal Control (IC), Tube PCR. |
Storage | 12 months, temperature -20oC |
User manual | TopSPEC® CSFV RT-qPCR KIT |
Above are detailed information about TopSPEC® CSFV RT-qPCR KIT. In addition, we also have other test kits such as: Babesia, ASFV, FMDV, H5N1, etc.
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ABT Equipment Co., Ltd. is always confident and proud of our staff with years of experience in molecular biology, especially test kits related to viral and bacterial diseases in humans, food, aquatic, and veterinary. If you have any questions or need help, please contact: