INTRODUCTION
Japanese encephalitis is not only a disease in humans but also affects mammals, especially cattle such as pigs, cows, buffaloes, etc. After the virus enters the body, they enter the bloodstream and move to the internal organs. Finally, when it reaches the developmental stage, encephalitis virus will cause damage to the liver, kidneys, spleen, muscles, and eventually the central nervous system. Infected subjects often have poor immune systems. In particular, when the mother is infected, there is a very high rate of miscarriage. The disease is contagious at any age, therefore early detection will be the most effective risk prevention measure for farmers.
TopSPEC® JEV RT-qPCR Kit is designed for the rapid and accurate detection of Japanese encephalitis virus on various sample type (rectal smears, stool and jejunal tissue samples, lip samples) farm) by Real-time PCR. The average time for the complete process of detection is 2 hours. The kit is a combination of many modern technologies in molecular biology to significantly improve the specificity and sensitivity of the reaction compared to other products on the market today. In addition, during extraction or mixing, exogenous controls can be added to limit false negatives and manipulation errors.
Advantage
- Easy to use
- PCR time optimized for 2 hours
- Various types of samples
- High sensitivity and specificity
- Provide additional mastermix for control (75 tests)
- Compatible with most Realtime PCR machines on the market: Rotor Gene Q (Qiagen), 7500, 7500 Fast (Thermo Fisher), AriaMX, Mx 3005p, Bioer, Dlab, etc.
Specification
Target | Japanese encephalitis (JEV) |
Sample | RNA after extraction from rectal smear, stool, and jejunal tissue samples, and environmental samples. |
Volume | 5µL |
Channel | FAM: for JEV
HEX: for internal control |
Technology | TaqMan probe |
PCR time | 2 hours |
Specificity | only detect JEV |
Components | JEV rRT-PCR mix, Negative control, Positive control, Internal control (IC), Tube PCR. |
Storage | 12 months, temperature -20oC |
User manual | TopSPEC® JEV RT-qPCR KIT |
Above are detailed information about TopSPEC® JEV RT-qPCR KIT (SQV-109). In addition, we also have other test kits such as: Babesia, ASFV, FMDV, H5N1, etc.
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ABT Equipment Co., Ltd. is always confident and proud of our staff with years of experience in molecular biology, especially test kits related to viral and bacterial diseases in humans, food, aquatic, and veterinary. If you have any questions or need help, please contact: