INTRODUCTION
Porcine Epidemic Diarrhea (PED) is caused by the virus enter into the gastrointestinal tract, attacking and reducing the activity of the digestive tamarind. The virus exists for a long time in the livestock environment and spread through secretions and feces. Infected pigs will show signs of bleeding, indigestion, vomiting, and diarrhea. The disease spreads quickly and causes economic losses to farmers. Early detection helps to save costs and prevent losses caused by PED virus.
The TopSPEC® PED RT-qPCR Kit is designed for the rapid and accurate detection of ASF virus on various sample types (feces, whole blood, serum, environmental samples) by Real-time PCR. The average time for the complete process of detection is 2 hours. The kit is a combination of many modern technologies in molecular biology to significantly improve the specificity and sensitivity of the reaction compared to other products on the market today. In addition, during extraction or mixing, exogenous controls can be added to limit false negatives and manipulation errors.
Advantage
- Easy to use
- PCR time optimized for 2 hours
- Various types of samples
- High sensitivity and specificity
- Provide additional mastermix for control (75 tests)
- Compatible with most Realtime PCR machines on the market: Rotor Gene Q (Qiagen), 7500, 7500 Fast (Thermo Fisher), AriaMX, Mx 3005p, Bioer, Dlab, etc.
Specification
Target | Porcine Epidemic Diarrhea (PED) |
Sample | RNA after extraction from rectal swab samples, stool and jejunal tissue samples, and barn environment samples. |
Volume | 5µL |
Channel | FAM: for PED
HEX: for internal control |
Technology | TaqMan probe |
PCR time | 2 hours |
Specificity | only detect PED |
Components | PED rRT-PCR mix, Negative control, Positive control, Internal control (IC), Tube PCR. |
Storage | 12 months, temperature -20oC |
User manual | TopSPEC® PED RT-qPCR KIT |
Above are detailed information about TopSPEC® PED RT-qPCR KIT. In addition, we also have other test kits such as: Babesia, ASFV, FMDV, H5N1, etc.
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ABT Equipment Co., Ltd. is always confident and proud of our staff with years of experience in molecular biology, especially test kits related to viral and bacterial diseases in humans, food, aquatic, and veterinary. If you have any questions or need help, please contact: